Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice (2024)

Lesscher, H. M. B., Bailey, A., Burbach, J. P. H., Van Ree, J. M., Kitchen, I., & Gerrits, M. A. F. M. (2003). Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice. European Journal of Neuroscience, 17(5), 1006-12.

Lesscher, Heidi M B ; Bailey, Alexis ; Burbach, J Peter H et al. / Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice. In: European Journal of Neuroscience. 2003 ; Vol. 17, No. 5. pp. 1006-12.

@article{011c2abd49cf4e26a46ed08b054e645f,

title = "Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice",

abstract = "Chronic treatment with the opioid antagonist naltrexone induces functional supersensitivity to opioid agonists, which may be explained by receptor up-regulation induced by opioid receptor blockade. In the present study, the levels of opioid receptor subtypes through the brain of mice were determined after chronic naltrexone treatment using quantitative in vitro autoradiography. This is the first complete mapping study in mice for micro-, delta- and kappa-opioid receptors after chronic naltrexone exposure. Treatment with naltrexone clearly induced up-regulation of micro- (mean 80%) and, to a lesser extent, delta-opioid receptors (mean 39%). The up-regulation of micro- and delta-opioid receptors was evident throughout the brain, although there was variation in the percentage change across brain regions. In contrast, consistent up-regulation of kappa-opioid receptors was observed in cortical structures only and was not so marked as for micro- and delta-opioid receptors. In noncortical regions kappa-opioid receptor expression was unchanged. Taken together, the present findings suggest opioid receptor subtype-selective regulation by chronic naltrexone treatment in mice.",

keywords = "Animals, Autoradiography, Brain, Image Processing, Computer-Assisted, Male, Mice, Naltrexone, Narcotic Antagonists, Receptors, Opioid, Receptors, Opioid, delta, Receptors, Opioid, kappa, Receptors, Opioid, mu, Signal Transduction, Time Factors, Up-Regulation",

author = "Lesscher, {Heidi M B} and Alexis Bailey and Burbach, {J Peter H} and {Van Ree}, {Jan M} and Ian Kitchen and Gerrits, {Mirjam A F M}",

year = "2003",

language = "English",

volume = "17",

pages = "1006--12",

journal = "European Journal of Neuroscience",

issn = "0953-816X",

publisher = "Blackwell Publishing Ltd",

number = "5",

}

Lesscher, HMB, Bailey, A, Burbach, JPH, Van Ree, JM, Kitchen, I & Gerrits, MAFM 2003, 'Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice', European Journal of Neuroscience, vol. 17, no. 5, pp. 1006-12.

Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice. / Lesscher, Heidi M B; Bailey, Alexis; Burbach, J Peter H et al.
In: European Journal of Neuroscience, Vol. 17, No. 5, 2003, p. 1006-12.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice

AU - Lesscher, Heidi M B

AU - Bailey, Alexis

AU - Burbach, J Peter H

AU - Van Ree, Jan M

AU - Kitchen, Ian

AU - Gerrits, Mirjam A F M

PY - 2003

Y1 - 2003

N2 - Chronic treatment with the opioid antagonist naltrexone induces functional supersensitivity to opioid agonists, which may be explained by receptor up-regulation induced by opioid receptor blockade. In the present study, the levels of opioid receptor subtypes through the brain of mice were determined after chronic naltrexone treatment using quantitative in vitro autoradiography. This is the first complete mapping study in mice for micro-, delta- and kappa-opioid receptors after chronic naltrexone exposure. Treatment with naltrexone clearly induced up-regulation of micro- (mean 80%) and, to a lesser extent, delta-opioid receptors (mean 39%). The up-regulation of micro- and delta-opioid receptors was evident throughout the brain, although there was variation in the percentage change across brain regions. In contrast, consistent up-regulation of kappa-opioid receptors was observed in cortical structures only and was not so marked as for micro- and delta-opioid receptors. In noncortical regions kappa-opioid receptor expression was unchanged. Taken together, the present findings suggest opioid receptor subtype-selective regulation by chronic naltrexone treatment in mice.

AB - Chronic treatment with the opioid antagonist naltrexone induces functional supersensitivity to opioid agonists, which may be explained by receptor up-regulation induced by opioid receptor blockade. In the present study, the levels of opioid receptor subtypes through the brain of mice were determined after chronic naltrexone treatment using quantitative in vitro autoradiography. This is the first complete mapping study in mice for micro-, delta- and kappa-opioid receptors after chronic naltrexone exposure. Treatment with naltrexone clearly induced up-regulation of micro- (mean 80%) and, to a lesser extent, delta-opioid receptors (mean 39%). The up-regulation of micro- and delta-opioid receptors was evident throughout the brain, although there was variation in the percentage change across brain regions. In contrast, consistent up-regulation of kappa-opioid receptors was observed in cortical structures only and was not so marked as for micro- and delta-opioid receptors. In noncortical regions kappa-opioid receptor expression was unchanged. Taken together, the present findings suggest opioid receptor subtype-selective regulation by chronic naltrexone treatment in mice.

KW - Animals

KW - Autoradiography

KW - Brain

KW - Image Processing, Computer-Assisted

KW - Male

KW - Mice

KW - Naltrexone

KW - Narcotic Antagonists

KW - Receptors, Opioid

KW - Receptors, Opioid, delta

KW - Receptors, Opioid, kappa

KW - Receptors, Opioid, mu

KW - Signal Transduction

KW - Time Factors

KW - Up-Regulation

M3 - Article

C2 - 12653976

SN - 0953-816X

VL - 17

SP - 1006

EP - 1012

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

IS - 5

ER -

Lesscher HMB, Bailey A, Burbach JPH, Van Ree JM, Kitchen I, Gerrits MAFM. Receptor-selective changes in mu-, delta- and kappa-opioid receptors after chronic naltrexone treatment in mice. European Journal of Neuroscience. 2003;17(5):1006-12.